Human Pluripotent Stem Cell facility at NPSC
Facility provides hESC and hiPSC cell lines, culture media, genome editing (CRISPR), transfection tools, and reprogramming services for stem cell research lab
Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to differentiate into every cell type in the human body. They provide a powerful tool for life scientists and medical researchers in diverse fields such as cell and developmental biology, drug discovery, and regenerative medicine. The facility helps researchers to integrate the use of hESCs and hiPSCs into their work and provides training / reagents to staff and students using them.
Reagents and services provided include:
- hESC cell lines
- hiPSC cell lines
- human pluripotent stem cell culture medium and extracellular matrix
- transfection, stable cell lines, and genome editing (siRNA and mRNA transfection, piggyback transposons, CRISPR/Cas9 editing using RNP complexes).
- production of hiPSC lines using mRNA reprogramming
Facility pricing
Project costs are dependent on the amount of time and reagents required. Indicative costs are below but users should be aware that this will vary depending on the specific work. Prices will change annually at least in-line with inflation.
Projects can be customised according to your requirements. Get in touch to discuss with Lindsay to discuss your specific needs.
| HPSC Reagents Price List | ||
| Reagent | Unit | Cost |
| TESR | 500ml | £ 190.61 |
| bFGF | 0.5 ml / 30 micro g/ml | £ 2.57 |
| Noggin | 0.5 ml / 10 micro g/ml | £ 29.53 |
| Y27632 | 0.25 ml / 10 mM | £ 6.18 |
| Geltrex | 2ml / 2 mg/ml | £ 2.88 |
| Neon tips | 1 each | £ 22.27 |
| Service Provision | Cost | Notes | |||
| HPSC Services (staff time) | £77.29 | Per hour | |||
| Monthly access charge | ~£30/group/month | Applicable every month, charge may vary slightly and covers TC plastics, waste disposal, liquid nitrogen etc. | |||
| Thawing, expansion, freezing and QC | £ 557.49 | Per cell line, to establish culture of group specific cell line | |||
| Preparation of CRISPR KO / KI cell lines (typically 5 clones frozen) | £ 1,335.53 | Per cell line | |||
Facility staff
- Type
- person
Dr Lindsay Davidson
- Type
- person
Dr Margarita Kalamara
- Type
- person