Dr Jorunn Bos
Plant Sciences, School of Life Sciences
+44 (0)1382 385400
James Hutton Institute
My group aims to understand how aphids and plants interact with each other at the molecular level. More specifically, we try to dissect the molecular basis of plant immunity and susceptibility, and functionally characterize aphid molecules, called effectors, that contribute to aphid virulence.
Aphids are phloem-feeding insects that cause feeding damage and transmit economically important plant viruses to many crops worldwide. These insects form close associations with their hosts and use specialized mouthparts (stylets), to probe leaf tissue and feed from the phloem. The stylets follow a mainly extracellular route through the different plant cell layers to reach the phloem, and puncture cells along the pathway. During probing and feeding, saliva is secreted, which is rich in proteins and small molecules. The secreted proteins in saliva are delivered inside plant cells and the extracellular space where they are involved in the manipulation of host cellular processes to benefit the infestation process. We use a combination of large-scale (“omics”) approaches to look at the transcriptional and proteome changes that take place in both plants and aphids during interactions. In addition we are interested in understanding the functions of salivary proteins that function as effectors. More information on our main projects and the tools we use is detailed below.
Our work contributes to the development of novel strategies to control infestations by aphids and potentially other pests and pathogens, thereby improving crop yields and food security both in the UK and elsewhere, including in developing countries.
Characterization of plant and aphid responses during host and non-host interactions
Many aphid species are restricted to one or few host plants, while some aphids, many of which are of agricultural importance, can infest a wide range of plant species. An important observation is that aphids spend a considerable time on non-host species, where they probe the leaf tissue and secrete saliva, but for unknown reasons are unable to ingest phloem sap. These findings suggest that aphids, like plant pathogens, interact with non-host plants at the molecular level, but potentially are not successful in suppressing plant defenses and/or releasing nutrients. We are interested investigating the molecular changes in both plant and aphids during host and non-host interactions. We use a range of "omics" approaches to gain insight into both plant and aphid genes and proteins involved in determining host range.
Characterization of aphid effectors and their host targets
To gain insight into the mode-of-action of aphid effectors we aim to characterize their role in host cell manipulation. We use a combination of approaches including confocal microscopy, yeast-2-hybrid screening, biochemistry approaches, as well as functional assays in different plant species to achieve this aim. Effector localisation work has identified different intracellular localisation into membranes, nuclei and cytoplasm. In addition, we use proteomics and yeast-2-hybrid screens to identify plant targets of candidate effectors and elucidate mechanisms of host cell manipulation. To study effector and host target contribution to virulence we use a combination of in planta overexpression and silencing and aphid performance assays.
Our work recently led to the first report of an aphid effector-host protein interaction. We showed that aphid effector Mp1 interacts with a host protein involved in plant cell trafficking. The ability of the effector to interact with this host protein was linked to its virulence activity. Also, aphid infestation leads to degradation of the host protein indicating it is a potentially important virulence target.
Figure legend: Aphid mouthparts (or stylets) penetrate the leaf surface and follow a mainly intercellular pathway to reach the phloem. Most cells along this pathway are punctured by the aphid stylets. Saliva is secreted along the intercellular stylet pathway, into the different cell types along the stylet pathway, and into the phloem. This saliva contains proteins that are predicted to function as effectors through interacting with and/or modifying host proteins. These events likely result in suppression of host defenses and a change of host physiology to promote nutrient uptake.
Review of the Year 2021 took place virtually today with the former Interim Dean, Inke Näthke sharing the highlights of activity within the School from 2021
Crop genomics for food security, sustaining human and environmental health
The public are invited to discover the wonderful world of plants and the people who work with them at an upcoming event at the University of Dundee Botanic Garden
|Brian Cox Prize: Engaged Researcher||2021|
|Major Personal Funding Awards / ERC Consolidator Grant||2020|
|Major Personal Funding Awards / European Research Council Starting Grant||2012|