Dr Alan Prescott
Cell Signalling and Immunology, School of Life Sciences
+44 (0)1382 384884
James Black Centre
Macropinocytosis by dendritic cells
Dendritic cells are essential for presentation of foreign antigens to T cells to illicit an immune response. They sample their peripheral tissue environment by engulfing large volumes of the surrounding mileu by macropinocytosis. Macropinosomes form from the aggregation of many actin-driven ruffles. This process is down regulated in mature dendritic cells which migrate to secondary lymphoid organs for antigen presentation to T cells. During the initial encounter with antigen there is a brief burst of enhanced macropinocytosis accompanied by a loss of podosomes-cell substrate adhesive structures that are also actin rich. The main thrust of our research is to understand how these two types of actin structures are regulated. Novel regulators of the actin cytoskeleton will be identified and localised by advanced microscopy techniques. (Collaborative project with Colin Watts)
See Fig. A. below - Dendritic cell labelled for F-actin with phalloidin. Projection of a z-series colour coded for depth. Membrane ruffles associated with macropinosome formation rise up from the surface of the cell (coloured green and blue). Podosomes associated with the base of the cell near the substrate are coloured orange.
See Fig B/C below - Scanning electron micrograph of a dendritic cell. C. Detail of B showing cell surface immuno-gold labelling (red) with MHC class I antibody.
See Fig D below - Dendritic cells are the sentinels of the immune system, residing in peripheral tissues, constantly sampling their environment for invading pathogens. The image shows a 3D reconstruction of DCs in the sub-mucosal layers of the small intestine colour-coded for depth. On the left is the view from the front, on the right the rear view.
In the summer of 2019, undergraduate student Fergus Buchanan undertook a lab project with Dr Alan Prescott.
Alan Prescott has been chosen to be the regional (Europe) EMEA winner of the Olympus Image of the Year Award 2019. This was for the submitted image with the title "The mouse’s whiskers: image from a frozen section of the head from the mito-QC mouse."
This week forty researchers completed a two-day workshop in Cryo Electron Microscopy (EM) held in the School.
The role of the cytoskeleton in cell specific differentiation
|Fellows of the Royal Society of Biology||2011|