David Horn lab resources - Stem loop RNAi cassettes
Cassette sequenced in full, February 2008
Two versions are available, containing different multiple cloning sites separated by a 468 bp stuffer (pBluescript 117-585, including 125 bp of the β-galactosidase α-fragment)
All of the restriction sites listed are unique.
MCS1/2 KpnI SmaI AflII BamHI 1 GGTACCCGGG CTTAAGGATC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG 51 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA 101 AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA 151 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG 201 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA 251 AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA 301 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT 351 ACAGGGCGCG TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 401 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 451 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCTAG AAGCTTCTCG 501 AGGGCCC XbaI HindIII XhoI ApaI MCS3/4 KpnI AgeI ClaI MfeI SmaI Note: AgeI & XmaI (isoschizomer of SmaI) give compatible overhangs;
ClaI site blocked by Dam methylation 1 GGTACCGGTA TCGATCAATT GCCCGGGTCC GAGATAGGGT TGAGTGTTGT 51 TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA 101 AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA 151 CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA 201 CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG 251 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG 301 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA 351 TGCGCCGCTA CAGGGCGCGT CCATTCGCCA TTCAGGCTGC GCAACTGTTG 401 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG 451 GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCAGAT 501 CTTAAGGCCG GCCTCGAGTC TAGAGGGCCC BglII AflII FseI XhoI XbaI ApaI