David Horn lab publications
Read publications for the David Horn lab
Genetic origins and proteomic consequences of kinetoplast loss in trypanosomes. Ridgway M, Escrivani DO, Novotná M, Wood A, Tinti M, Schnaufer A, Horn D. (2026) PLoS Pathogens. 22:e1013846. Peer Review file
Acoziborole resistance associated mutations in Trypanosoma brucei CPSF3. Ridgway M, Novotná M, Mendoza Martinez C, Tinti M, Altmann S, Sloan G, Horn D. (2026) PLoS Pathogens. 22:e1013764. Peer Review file. Featured in Nature Portfolio Microbiology Community.
Decoding efficacy and resistance space at a drug binding site. Altmann S, Mendoza Martinez C, Ridgway M, Tinti M, Saini JS, Ibrahim PEGF, Thomas M, De Rycker M, Bodkin MJ, Horn D. (2026) Nat Communications 17:2337. Peer Review file. Featured in Nature Portfolio Microbiology Community. FLS news. LinkedIn. Related video.
A non-coding role for trypanosome VSG transcripts in allelic exclusion. Escrivani DO, Hutchinson S, Tinti M, Wright JE, Marques CA, Faria JRC, Trenaman A, Horn D. (2025) Nucleic Acids Research. 53(19). FLS news.
Precision-edited histone tails disrupt polycistronic gene expression controls in trypanosomes. Novotná M, Tinti M, Faria JRC, Horn, D. (2025) Nature Communications 16:6194. Peer Review file. FLS news.
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes. Trenaman A, Tinti M, Wall RJ, Horn D. (2024) Nature Communications. 15:8113. Peer Review file. FLS news.
An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome. Faria JRC, Tinti M, Marques CA, Zoltner M, Yoshikawa H, Field MC, Horn D. (2023) Nature Communications. 14:8200. Peer Review file. Featured in Nature Portfolio Microbiology Community. FLS news.
Competition among variants is predictable and contributes to the antigenic variation dynamics of African trypanosomes. Escrivani DO, Scheidt V, Tinti M, Faria J, Horn D. (2023) PLoS Pathog. 19:e1011530. Peer Review file.
Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defects. Marques CA, Ridgway M, Tinti M, Cassidy A and Horn D (2022) Nature Communications. 13:5326. Peer Review file. FLS news.
Oligo targeting for profiling drug resistance mutations in the parasitic trypanosomatids. Altmann S, Rico E, Carvalho S, Ridgway M, Trenaman A, Donnelly H, Tinti M, Wyllie S and Horn D (2022) Nucleic Acids Res. 50:e79. Related video. FLS news.
Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes. Faria J, Luzak V, Müller LSM, Brink BG, Hutchinson S, Glover L, Horn D, Siegel TN. (2021) Nature Microbiology. 6:289-300. Peer Review file. Featured in Nat Rev Microbiol News & Analysis, Nat Microbiol editorial, preLights, Nature Portfolio Microbiology Community. FLS news.
Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex. Faria J, Glover L, Hutchinson S, Boehm C, Field MC, Horn D. (2019) Nature Communications. 10:3023. Peer Review file.
A post-transcriptional respiratome regulon in trypanosomes. Trenaman A, Glover L, Hutchinson S, Horn D. (2019) Nucleic Acids Res. 47:7063-7077.
Gluconeogenesis using glycerol as a substrate in bloodstream-form Trypanosoma brucei. Kovářová J, Nagar R, Faria J, Ferguson MAJ, Barrett MP, Horn D. (2018) PLoS Pathog. 14:e1007475 PLoS Pathogens Press Release.
Clinical and veterinary trypanocidal benzoxaboroles target CPSF3. Wall RJ, Rico E, Lukac I, Zuccotto F, Elg S, Gilbert IH, Freund Y, Alley MRK, Field MC, Wyllie S, Horn D. (2018) Proc. Nat’l Acad. Sci. USA. 115:9616-9621
Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes. Rico E, Kovářová J, Jeacock L and Horn D. (2018) Scientific Reports. 8:7960
Codon usage bias controls mRNA and protein abundance in trypanosomatids. Jeacock L, Faria J and Horn D. (2018) eLife. 7:e32496. Peer Review file. Featured in Trends in Parasitology.
Aquaglyceroporin-null trypanosomes display glycerol transport defects and respiratory-inhibitor sensitivity. Jeacock L, Baker N, Wiedemar N, Mäser P and Horn D. (2017) PLoS Pathogens. 13:e1006307
VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes. Glover L, Hutchinson S, Alsford S and Horn D. (2016) Proc. Natl. Acad. Sci. USA. 113:7225-30 Featured in Proc. Natl. Acad. Sci. USA Commentary; Drug Discovery Today.
Vacuolar ATPase depletion affects mitochondrial ATPase function, kinetoplast dependency and drug-sensitivity in trypanosomes. Baker N, Hamilton G, Wilkes J, Hutchinson S, Barrett MP, Horn D. (2015) Proc. Natl. Acad. Sci. USA. 112:9112-9117
Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei. Alsford S, Currier RB, Guerra-Assunção JA, Clark TG, Horn D (2014) PLoS Pathogens. 10:e1004130
Locus-specific control of DNA resection and suppression of subtelomeric VSG recombination by HAT3 in the African trypanosome. Glover L, Horn D (2014) Nucleic Acids Res. 42:12600-12613
DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. Glover L, Alsford S and Horn D (2013) PLoS Pathogens 9:e1003260
Cell cycle regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei. Alsford S and Horn D (2012) Nucleic Acids Res. 40:10150-60
Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. Baker N, Glover L, Munday JC, Aguinaga Andrés D, Barrett MP, de Koning HP and Horn D (2012) Proc. Natl. Acad. Sci. USA 109:10996–11001 Featured in : LSHTM news; WHO policy document; Dundee student video.
High-throughput decoding of antitrypanosomal drug efficacy and resistance. Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung K, Turner DJ, Field MC, Berriman M and Horn D. (2012) Nature 482:232-236 PMC Featured in: Nature News & Views, New Scientist, Pathogens & Global Health, LSHTM news, WHO policy document, Other media.
Elongator protein 3b negatively regulates ribosomal DNA transcription in African trypanosomes. Alsford S and Horn D. (2011) Mol Cell Biol 31:1822-1832
Microhomology mediated deletion and gene conversion in African trypanosomes. Glover L, Jun J and Horn D. (2011) Nucleic Acids Res. 39:1372-1380
High throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Alsford S, Turner DJ., Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C and Horn D. (2011) Genome Research 21:915-924 Featured in: Nature Biotechnology, Nature Methods, Nature Reviews Microbiology. Data hosted by: Tritryp Database, TDR Targets Database, iNTRODB.
FMOPhore for hotspot identification and efficient fragment-to-lead growth strategies. Ibrahim PEGF, Altmann S, Zachariae U, Horn D, Gilbert IH, Bodkin MJ. Nat Commun. 2026 Apr 28.
Identification of a proteasome-targeting arylsulfonamide with potential for the treatment of Chagas’ disease. Lima M, Tulloch L, Corpas Lopez V, Carvalho S, Wall R, Milne R, Rico E, Patterson S, Gilbert I, Moniz S, MacLean L, Torrie L, Morgillo C, Horn D, Zuccotto F, Wyllie S. (2022) Antimicrob. Agents Chemother. 66:e0153521.
Veterinary trypanocidal benzoxaboroles are peptidase-activated prodrugs. Giordani F, Paape D, Vincent IM, Pountain AW, Fernandez-Cortes F, Rico E, Zhang N, Morrison LJ, Freund Y, Witty MJ, Peter R, Edwards DY, Wilkes J, Van der Hooft J, Regnault C, Read KD, Horn D, Field MC, Barrett MP. (2020) PLoS Pathogens. e1008932. Peer Review file.
Preclinical candidate for the treatment of visceral leishmaniasis that acts through proteasome inhibition. Wyllie S, Brand S, Thomas M, De Rycker M, Chung CW, Pena I, Bingham RP, Bueren-Calabuig JA, Cantizani J, Cebrian D, Craggs PD, Ferguson L, Goswami P, Hobrath J, Howe J, Jeacock L, Ko EJ, Korczynska J, MacLean L, Manthri S, Martinez MS, Mata-Cantero L, Moniz S, Nühs A, Osuna-Cabello M, Pinto E, Riley J, Robinson S, Rowland P, Simeons FRC, Shishikura Y, Spinks D, Stojanovski L, Thomas J, Thompson S, Viayna Gaza E, Wall RJ, Zuccotto F, Horn D, Ferguson MAJ, Fairlamb AH, Fiandor JM, Martin J, Gray DW, Miles TJ, Gilbert IH, Read KD, Marco M, Wyatt PG. (2019) Proc Natl Acad Sci U S A. 116:9318-9323. See Wellcome Trust video.
Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei. Collett CF, Kitson C, Baker N, Steele-Stallard HB, Santrot MV, Hutchinson S, Horn D, Alsford S. (2019) Antimicrob Agents Chemother. e00795-19.
Antitrypanosomal 8-hydroxy-naphthyridines are chelators of divalent transition metals. Wall RJ, Moniz S, Thomas MG, Norval S, Ko EJ, Marco M, Miles TJ, Gilbert IH, Horn D, Fairlamb AH, Wyllie S. (2018) Antimicrob Agents Chemother. e00235-18. GlaxoSmithKline Scientific Termination of Projects (STOP) Award 2017
Host-Parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles. Zhang N, Zoltner M, Leung K-F, Scullion P, Hutchinson S, del Pino RC, Vincent IM, Zhang Y-K, Freund YR, Alley MRK, Jacobs RT, Read KD, Barrett MP, Horn D and Field MC. (2018) PLoS Pathogens. 14:e1006850.
Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs. Meredith EL, Kumar A, Konno A, Szular J, Alsford S, Seifert K, Horn D and Wilkinson SR. (2017) Mol. Microbiol. 106:207-222. Featured in Mol. Microbiol. MicroCommentary
Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei. Stortz JA, Serafim TD, Alsford S, Wilkes J, Fernandes-Cortes F, Hamilton G, Briggs E, Lemgruber L, Horn D, Mottram JC and McCulloch R. (2017) PLoS Pathogens.13:e1006477
Genome-wide RNAi selection identifies a regulator of transmission stage-enriched gene families and cell-type differentiation in Trypanosoma brucei. Rico E, Ivens A, Glover L, Horn D and Matthews K. (2017) PLoS Pathogens. 13:e1006279
The role of folate transport in antifolate drug action in Trypanosoma brucei. Dewar S, Sienkiewicz N, Ong HB, Wall RJ, Horn D and Fairlamb AH. (2016) J. Biol. Chem. 291:24768–24778
Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression. Maishman L, Obado SO, Alsford S, Bart J, Chen W, Ratushny AV, Navarro M, Horn D, Aitchison JD, Chait BT, Rout MP and Field MC. (2016) Nucleic Acids Res. 44:10554-10570
Pentamidine is not a permeant but a nanomolar inhibitor of the Trypanosoma brucei aquaglyceroporin-2. Song J, Baker N, Rothert M, Henke B, Jeacock L, Horn D, Beitz E. (2016) PLoS Pathogens. 12:e1005436
Modulation of the surface proteome through multiple ubiquitylation pathways in African trypanosomes. Zoltner M, Leung K-F, Alsford S, Horn D and Field MC. (2015) PLoS Pathogens. 11:e1005236
Chimerization at the AQP2-AQP3 locus is the genetic basis of melarsoprol-pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates. Graf FE, Baker N, Munday JC, de Koning HP, Horn D, Mäser P. (2015) Int. J. Parasitol; Drugs & Drug Resistance 5:65-68
Genome wide dissection of the quorum sensing signaling pathway in Trypanosoma brucei. Mony BM, MacGregor P, Ivens A, Rojas F, Cowton A, Wilson J, Horn D and Matthews KR (2014) Nature. 505:681-685
Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNAi library screening for resistance to the phosphodiesterase inhibitor Cpd A. Gould MK, Bachmaier S, Ali JA, Alsford S, Tagoe DN, Munday JC, Schnaufer AC, Horn D, Boshart M and de Koning HP (2013) Antimicrob. Agents Chemother. 57:4882-93
PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome. Rudd SG, Glover L, Jozwiakowski SK, Horn D and Doherty AJ (2013) Mol. Cell 52:554-565. See Nature Struct. & Mol. Biol. News & Views.
NUP-1 Is a large coiled-coil nucleoskeletal protein in trypanosomes with lamin-like functions. duBois K, Alsford S, Buisson J, Swiderski M, Bart J, Bastin P, Barry D, Navarro M, Horn D, Aitchison J, Rout M and Field M. (2012) PLOS Biology 10:e1001287
Pass the boron: benzoxaboroles as antiparasite drugs. Zoltner M, Horn D, Field MC. (2024) Trends Parasitol. 40:820-828.
Anti-trypanosomatid drug discovery: progress and challenges. De Rycker M, Wyllie S, Horn D, Read KD, Gilbert IH. (2023) Nature Reviews Microbiology. 21;35-50.
A profile of research on the parasitic trypanosomatids and the diseases they cause. Horn D. (2022) PLoS Negl Trop Dis. 16:e0010040
Genome-scale RNAi screens in African trypanosomes Horn D. (2022) Trends Parasitol. 38:160-173 Featured in Cell Press special collection for World NTDs Day 2022
Melarsoprol resistance in African trypanosomiasis. Fairlamb, A and Horn, D. (2018) Trends in Parasitology. 34:481-492
Anti-trypanosomatid drug discovery: An ongoing challenge and a continuing need. Field MC, Horn D, Fairlamb AH, Ferguson MAJ, Gray DW, Read KD, De Rycker M, Torrie LS, Wyatt PG, Wyllie S and Gilbert IH. (2017) Nature Reviews Microbiol. 15:217-231
Research Matters: How antiparasitic drugs work – and sometimes stop working! * Horn D. (2016) PLoS Pathogens. 12:e1005430
Protocol: Genome-scale RNAi screens for high-throughput phenotyping in bloodstream form African trypanosomes. Glover L, Alsford S, Baker N, Turner DJ, Sanchez-Flores A, Hutchinson S, Hertz-Fowler C, Berriman M and Horn D (2015) Nature Protocols. 10:106–133
Antigenic variation in African trypanosomes. Horn D. (2014) Mol. Biochem. Parasitol. 195:123-129
2026 – preprints under review
ZNK1 senses zinc and degrades zinc transporter mRNA in trypanosomes. Teresa Leao, Anna Trenaman, Michele Tinti, Gustavo Bravo Ruiz, Idalio Viegas, Luisa M Figueiredo, Margarida Duarte, Ana Tomas, David Horn. https://doi.org/10.64898/2026.01.27.702079 BioRxiv
Thermo-sensing and argonaute-dependent transcriptome remodelling in trypanosomes. Gustavo Bravo Ruiz, Michele Tinti, David Horn. https://doi.org/10.64898/2026.01.20.700697 BioRxiv
Targeted protein degradation of the CPSF complex by benzoxaboroles through sumoylation. Ning Zhang, Abdul Samad, Farnaz Zahedifard, Richard J. Wall, David Horn, Martin Zoltner, Mark C. Field. doi: https://doi.org/10.1101/2024.03.11.584402 BioRxiv
2026
A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes. Miskinyte M, Jetishi C, Kalichava A, Ivens A, Waterfall M, Gould M, Glover L, Horn D, Ochsenreiter T, Schnaufer A. Nucleic Acids Res. 2026 May 20;54(10):gkag493. doi: 10.1093/nar/gkag493. PMID 42165136 Free article
FMOPhore for hotspot identification and efficient fragment-to-lead growth strategies. Ibrahim PEGF, Altmann S, Zachariae U, Horn D, Gilbert IH, Bodkin MJ. Nat Commun. 2026 Apr 28. doi: 10.1038/s41467-026-72384-x. PMID 42045222 Free article
Genetic origins and proteomic consequences of kinetoplast loss in trypanosomes. Ridgway M, Escrivani DO, Novotná M, Wood A, Tinti M, Schnaufer A, Horn D. PLoS Pathogens. 2026 Mar 25;22(3):e1013846. doi: 10.1371/journal.ppat.1013846. PMID 41880374 Free article
Acoziborole resistance associated mutations in Trypanosoma brucei CPSF3. Ridgway M, Novotná M, Mendoza Martinez C, Tinti M, Altmann S, Sloan G, Horn D. PLoS Pathogens. 2026 Mar 3;22(3):e1013764. doi: 10.1371/journal.ppat.1013764. PMID: 41774758 Free article
Decoding efficacy and resistance space at a drug binding site. Altmann S, Mendoza Martinez C, Ridgway M, Tinti M, Saini JS, Ibrahim PEGF, Thomas M, De Rycker M, Bodkin MJ, Horn D. Nat Commun. 2026. doi: 10.1038/s41467-026-69187-5. PMID: 41639120 Free article
2025
A non-coding role for trypanosome VSG transcripts in allelic exclusion. Escrivani DO, Hutchinson S, Tinti M, Wright JE, Marques CA, Faria JRC, Trenaman A, Horn D. Nucleic Acids Research. 2025 Oct 14;53(19): doi: 10.1093/nar/gkaf1011. PMID: 41118571 Free article
Decoding post-transcriptional gene expression controls in trypanosomatids using machine learning. Tinti M and Horn D. Wellcome Open Res. 2025, 10:173. https://wellcomeopenresearch.org/articles/10-173. PMID: 40735494 Free article
Precision-edited histone tails disrupt polycistronic gene expression controls in trypanosomes. Novotná M, Tinti M, Faria JRC, Horn D. Nat Commun. 2025 Jul 4;16(1):6194. doi: 10.1038/s41467-025-61480-z. PMID: 40615444 Free article
2024
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes. Trenaman A, Tinti M, Wall RJ, Horn D. Nat Commun. 2024 Sep 16;15(1):8113. doi: 10.1038/s41467-024-52432-0. PMID: 39285175 Free article.
Pass the boron: benzoxaboroles as antiparasite drugs. Zoltner M, Horn D, Field MC. Trends Parasitol. 2024 Sep;40(9):820-828. doi: 10.1016/j.pt.2024.07.003. Epub 2024 Aug 5. PMID: 39107181 Free article
The bloodstream form of Trypanosoma brucei displays non-canonical gluconeogenesis. Kovářová J, Moos M, Barrett MP, Horn D, Zíková A. PLoS Negl Trop Dis. 2024 Feb 23;18(2):e0012007. doi: 10.1371/journal.pntd.0012007. PMID: 38394337 Free PMC article.
Genome-wide screens connect HD82 loss-of-function to purine analog resistance in African trypanosomes. Trenaman A, Tinti M, Atrih A, Horn D. mSphere. 2023 Dec 21:e0036323. doi: 10.1128/msphere.00363-23. PMID: 38126788 Free PMC article.
2023
An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome. Faria JRC, Tinti M, Marques CA, Zoltner M, Yoshikawa H, Field MC, Horn D. Nature Communications. 2023 Dec 11;14(1):8200. doi: 10.1038/s41467-023-44043-y. PMID: 38081826 Free PMC article.
Competition among variants is predictable and contributes to the antigenic variation dynamics of African trypanosomes. Escrivani DO, Scheidt V, Tinti M, Faria J, Horn D. PLoS Pathog. 2023 Jul 17;19(7):e1011530. doi: 10.1371/journal.ppat.1011530. eCollection 2023 Jul. PMID: 37459347 Free PMC article.
Novel kinetoplastid-specific cAMP binding proteins identified by RNAi screening for cAMP resistance in Trypanosoma brucei. Bachmaier S, Gould MK, Polatoglou E, Omelianczyk R, Brennand AE, Aloraini MA, Munday JC, Horn D, Boshart M, de Koning HP. Front Cell Infect Microbiol. 2023 Jul 5;13:1204707. doi: 10.3389/fcimb.2023.1204707. eCollection 2023. PMID: 37475965 Free PMC article.
Anti-trypanosomatid drug discovery: progress and challenges. de Rycker M, Wyllie S, Horn D, Read KD, Gilbert IH. Nature Reviews Microbiology. 2023 Jan 21(1):35-50. doi: 10.1038/s41579-022-00777-y. PMID: 35995950 Free PMC article
2022
Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defects. Marques CA, Ridgway M, Tinti M, Cassidy A and Horn D. Nature Communications. 2022 Sep 10;13(1):5326. doi: 10.1038/s41467-022-33109-y. PMID: 36088375 Free PMC article
Oligo targeting for profiling drug resistance mutations in the parasitic trypanosomatids. Altmann S, Rico E, Carvalho S, Ridgway M, Trenaman A, Donnelly H, Tinti M, Wyllie S, Horn D. Nucleic Acids Res. 2022 Aug 12;50(14):e79. doi: 10.1093/nar/gkac319. PMID: 35524555 Free PMC article
Repositioning of a diaminothiazole series confirmed to target the cyclin-dependent kinase CRK12 for use in the treatment of African animal trypanosomiasis. Smith A, Wall RJ, Patterson S, Rowan T, Rico E, Stojanovski L, Huggett M, Hampton SE, Thomas MG, Corpas-Lopez V, Gillingwater K, Duke J, Napier G, Peter R, Vitouley HS, Harrison JR, Milne R, Jeacock L, Baker N, Davis SH, Simeons F, Riley J, Horn D, Brun R, Zuccotto F, Witty MJ, Wyllie S, Read KD, Gilbert IH. J. Med. Chem. 2022 Apr 14;65(7):5606-5624. doi: 10.1021/acs.jmedchem.1c02104. Epub 2022 Mar 18. PMID: 35303411 Free PMC article
CRISPR/Cas9-based precision tagging of essential genes in bloodstream form African trypanosomes. Kovářová J, Novotná M, Faria J, Rico E, Wallace C, Zoltner M, Field MC, Horn D. Molecular & Biochemical Parasitology. 2022 May;249:111476. doi: 10.1016/j.molbiopara.2022.111476. Epub 2022 Apr 1. PMID: 35378143 Free article
Control of variant surface glycoprotein expression by CFB2 in Trypanosoma brucei and quantitative proteomic connections to translation and cytokinesis. Bravo Ruiz G, Tinti M, Ridgway M, Horn D. mSphere. 2022 Apr 27;7(2):e0006922. doi: 10.1128/msphere.00069-22. Epub 2022 Mar 21. PMID: 35306877 Free article
A profile of research on the parasitic trypanosomatids and the diseases they cause. Horn D. PLoS Negl Trop Dis. 2022 Jan 13;16(1):e0010040. doi: 10.1371/journal.pntd.0010040. eCollection 2022 Jan. Review. PMID: 35025891 Free PMC article
Identification of a proteasome-targeting arylsulfonamide with potential for the treatment of Chagas’ disease. Lima M, Tulloch L, Corpas Lopez V, Carvalho S, Wall R, Milne R, Rico E, Patterson S, Gilbert I, Moniz S, MacLean L, Torrie L, Morgillo C, Horn D, Zuccotto F, Wyllie S. Antimicrob. Agents Chemother. 2022 Jan 18;66(1):e0153521. doi: 10.1128/AAC.01535-21. Epub 2021 Oct 4. PMID: 34606338 Free PMC article
Genome-scale RNAi screens in African trypanosomes. Horn D. Trends Parasitol. 2022 Feb;38(2):160-173. doi: 10.1016/j.pt.2021.09.002. Epub 2021 Sep 24. Review. PMID: 34580035
2021
Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes. Faria J, Luzak V, Müller LSM, Brink BG, Hutchinson S, Glover L, Horn D, Siegel TN. Nat Microbiol. 2021 Mar;6(3):289-300. doi: 10.1038/s41564-020-00833-4. PMID: 33432154 Free PMC Article
2020
Veterinary trypanocidal benzoxaboroles are peptidase-activated prodrugs. Giordani F, Paape D, Vincent IM, Pountain AW, Fernandez-Cortes F, Rico E, Zhang N, Morrison LJ, Freund Y, Witty MJ, Peter R, Edwards DY, Wilkes J, Van der Hooft J, Regnault C, Read KD, Horn D, Field MC, Barrett MP. PLoS Pathogens. 2020 Nov 3;16(11):e1008932. doi: 10.1371/journal.ppat.1008932. PMID: 33141865 Free PMC Article
Instability of aquaglyceroporin (AQP) 2 contributes to drug resistance in Trypanosoma brucei. Quintana JF, Bueren-Calabuig J, Zuccotto F, de Koning HP, Horn D, Field MC. PLoS Negl Trop Dis. 2020 Jul 9;14(7):e0008458. doi: 10.1371/journal.pntd.0008458. eCollection 2020 Jul. PMID: 32644992 Free PMC Article
Suramin exposure alters cellular metabolism and mitochondrial energy production in African trypanosomes. Zoltner M, Campagnaro GD, Taleva G, Burrell A, Cerone M, Leung KF, Achcar F, Horn D, Vaughan S, Gadelha C, Zíková A, Barrett MP, de Koning HP, Field MC. J Biol Chem. 2020 Apr 30. pii: jbc.RA120.012355. doi: 10.1074/jbc.RA120.012355. Epub 2020 Apr 30. PMID: 32354742 Free PMC Article
Setting our sights on infectious diseases. De Rycker M, Horn D, Aldridge B, Amewu RK, Barry CE 3rd, Buckner FS, Cook S, Ferguson MAJ, Gobeau N, Herrmann J, Herrling P, Hope W, Keiser J, Lafuente-Monasterio MJ, Leeson PD, Leroy D, Manjunatha UH, McCarthy J, Miles TJ, Mizrahi V, Moshynets O, Niles J, Overington JP, Pottage J, Rao SPS, Read KD, Ribeiro I, Silver LL, Southern J, Spangenberg T, Sundar S, Taylor C, Van Voorhis W, White NJ, Wyllie S, Wyatt PG, Gilbert IH. ACS Infect Dis. 2020 Jan 10;6(1):3-13. doi: 10.1021/acsinfecdis.9b00371. Epub 2019 Dec 6. Review. PMID: 31808676 Free PMC Article
2019
Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex. Faria J, Glover L, Hutchinson S, Boehm C, Field MC, Horn D. Nat Commun. 2019 Jul 9;10(1):3023. doi: 10.1038/s41467-019-10823-8. PMID: 31289266 Free PMC Article
Persistent DNA damage foci and DNA replication with a broken chromosome in the African trypanosome. Glover L, Marques CA, Suska O, Horn D. MBio. 2019 Jul 9;10(4). pii: e01252-19. doi: 10.1128/mBio.01252-19. PMID: 31289179 Free PMC Article
Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei. Collett CF, Kitson C, Baker N, Steele-Stallard HB, Santrot MV, Hutchinson S, Horn D, Alsford S. Antimicrob Agents Chemother. 2019 Jul 25;63(8). pii: e00795-19. doi: 10.1128/AAC.00795-19. PMID: 31160283 Free PMC Article
A post-transcriptional respiratome regulon in trypanosomes. Trenaman A, Glover L, Hutchinson S, Horn D. Nucleic Acids Res. 2019 Nucleic Acids Res. 2019 Jul 26;47(13):7063-7077. doi: 10.1093/nar/gkz455. PMID: 31127277 Free PMC Article
Preclinical candidate for the treatment of visceral leishmaniasis that acts through proteasome inhibition. Wyllie S, Brand S, Thomas M, De Rycker M, Chung CW, Pena I, Bingham RP, Bueren-Calabuig JA, Cantizani J, Cebrian D, Craggs PD, Ferguson L, Goswami P, Hobrath J, Howe J, Jeacock L, Ko EJ, Korczynska J, MacLean L, Manthri S, Martinez MS, Mata-Cantero L, Moniz S, Nühs A, Osuna-Cabello M, Pinto E, Riley J, Robinson S, Rowland P, Simeons FRC, Shishikura Y, Spinks D, Stojanovski L, Thomas J, Thompson S, Viayna Gaza E, Wall RJ, Zuccotto F, Horn D, Ferguson MAJ, Fairlamb AH, Fiandor JM, Martin J, Gray DW, Miles TJ, Gilbert IH, Read KD, Marco M, Wyatt PG. Proc Natl Acad Sci U S A. 2019 May 7;116(19):9318-9323. doi: 10.1073/pnas.1820175116. Epub 2019 Apr 8. PMID: 30962368 Free PMC Article
Pharmacological validation of N-myristoyltransferase as a drug target in Leishmania donovani. Corpas-Lopez V, Moniz S, Thomas M, Wall RJ, Torrie LS, Zander-Dinse D, Tinti M, Brand S, Stojanovski L, Manthri S, Hallyburton I, Zuccotto F, Wyatt PG, De Rycker M, Horn D, Ferguson MAJ, Clos J, Read KD, Fairlamb AH, Gilbert IH, Wyllie S. ACS Infect Dis. 2019 Jan 11;5(1):111-122. doi: 10.1021/acsinfecdis.8b00226. Epub 2018 Nov 12. PMID: 30380837 Free PMC Article
2018
Gluconeogenesis using glycerol as a substrate in bloodstream-form Trypanosoma brucei. Kovářová J, Nagar R, Faria J, Ferguson MAJ, Barrett MP, Horn D. PLoS Pathog. 2018 Dec 27;14(12):e1007475. doi: 10.1371/journal.ppat.1007475. eCollection 2018 Dec. PMID: 3058989 Free PMC Article
Insights into antitrypanosomal drug mode-of-action from cytology-based profiling. Thomas JA, Baker N, Hutchinson S, Dominicus C, Trenaman A, Glover L, Alsford S, Horn D. PLoS Negl Trop Dis. 2018 Nov 26;12(11):e0006980. doi: 10.1371/journal.pntd.0006980. eCollection 2018 Nov. PMID: 30475806 Free PMC Article
Clinical and veterinary trypanocidal benzoxaboroles target CPSF3. Wall RJ, Rico E, Lukac I, Zuccotto F, Elg S, Gilbert IH, Freund Y, Alley MRK, Field MC, Wyllie S, Horn D. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9616-9621. doi: 10.1073/pnas.1807915115. Epub 2018 Sep 5. PMID: 30185555 Free PMC Article
Faster growth with shorter antigens can explain a VSG hierarchy during African trypanosome infections: a feint attack by parasites. Liu D, Albergante L, Newman TJ, Horn D. Sci Rep. 2018 Jul 19;8(1):10922. doi: 10.1038/s41598-018-29296-8. PMID: 30026531 Free PMC Article
Antitrypanosomal 8-hydroxy-naphthyridines are chelators of divalent transition metals. Wall RJ, Moniz S, Thomas MG, Norval S, Ko EJ, Marco M, Miles TJ, Gilbert IH, Horn D, Fairlamb AH, Wyllie S. Antimicrob Agents Chemother. 2018 Jul 27;62(8). pii: e00235-18. doi: 10.1128/AAC.00235-18. Print 2018 Aug. PMID: 29844044 Free PMC Article
Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes. Rico E, Jeacock L, Kovářová J, Horn D. Sci Rep. 2018 May 21;8(1):7960. doi: 10.1038/s41598-018-26303-w. PMID: 29785042 Free PMC Article
Benzoxaborole treatment perturbs S-adenosyl-L-methionine metabolism in Trypanosoma brucei. Steketee PC, Vincent IM, Achcar F, Giordani F, Kim DH, Creek DJ, Freund Y, Jacobs R, Rattigan K, Horn D, Field MC, MacLeod A, Barrett MP. PLoS Negl Trop Dis. 2018 May 14;12(5):e0006450. doi: 10.1371/journal.pntd.0006450. eCollection 2018 May. PMID: 29758036 Free PMC Article
Melarsoprol resistance in African trypanosomiasis. Fairlamb AH, Horn D. Trends Parasitol. 2018 Jun;34(6):481-492. doi: 10.1016/j.pt.2018.04.002. Epub 2018 Apr 25. Review. PMID: 29705579 Free Article
Codon usage bias controls mRNA and protein abundance in trypanosomatids. Jeacock L, Faria J, Horn D. Elife. 2018 Mar 15;7. pii: e32496. doi: 10.7554/eLife.32496. PMID: 29543155 Free PMC Article
Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles. Zhang N, Zoltner M, Leung KF, Scullion P, Hutchinson S, Del Pino RC, Vincent IM, Zhang YK, Freund YR, Alley MRK, Jacobs RT, Read KD, Barrett MP, Horn D, Field MC. PLoS Pathog. 2018 Feb 9;14(2):e1006850. doi: 10.1371/journal.ppat.1006850. eCollection 2018 Feb. PMID: 29425238 Free PMC Article
2017
Distinct activation mechanisms trigger the trypanocidal activity of DNA damaging prodrugs. Meredith EL, Kumar A, Konno A, Szular J, Alsford S, Seifert K, Horn D, Wilkinson SR. Mol Microbiol. 2017 Oct;106(2):207-222. doi: 10.1111/mmi.13767. Epub 2017 Aug 31. PMID: 28792090 Free PMC Article
Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei. Stortz JA, Serafim TD, Alsford S, Wilkes J, Fernandez-Cortes F, Hamilton G, Briggs E, Lemgruber L, Horn D, Mottram JC, McCulloch R. PLoS Pathog. 2017 Jul 24;13(7):e1006477. doi: 10.1371/journal.ppat.1006477. eCollection 2017 Jul. PMID: 28742144 Free PMC Article
Ornithine uptake and the modulation of drug sensitivity in Trypanosoma brucei. Macedo JP, Currier RB, Wirdnam C, Horn D, Alsford S, Rentsch D. FASEB J. 2017 Oct;31(10):4649-4660. doi: 10.1096/fj.201700311R. Epub 2017 Jul 5. PMID: 28679527 Free PMC Article
Aquaglyceroporin-null trypanosomes display glycerol transport defects and respiratory-inhibitor sensitivity. Jeacock L, Baker N, Wiedemar N, Mäser P, Horn D. PLoS Pathog. 2017 Mar 30;13(3):e1006307. doi: 10.1371/journal.ppat.1006307. eCollection 2017 Mar. PMID: 28358927 Free PMC Article
Genome-wide RNAi selection identifies a regulator of transmission stage-enriched gene families and cell-type differentiation in Trypanosoma brucei. Rico E, Ivens A, Glover L, Horn D, Matthews KR. PLoS Pathog. 2017 Mar 23;13(3):e1006279. doi: 10.1371/journal.ppat.1006279. eCollection 2017 Mar. PMID: 28334017 Free PMC Article
Anti-trypanosomatid drug discovery: an ongoing challenge and a continuing need. Field MC, Horn D, Fairlamb AH, Ferguson MA, Gray DW, Read KD, De Rycker M, Torrie LS, Wyatt PG, Wyllie S, Gilbert IH. Nat Rev Microbiol. 2017 Feb 27;15(4):217-231. doi: 10.1038/nrmicro.2016.193. [Epub ahead of print] Review. PMID: 28239154
2016
Exploiting the Achilles’ heel of membrane trafficking in trypanosomes. Zoltner M, Horn D, de Koning HP, Field MC. Curr Opin Microbiol. 2016 Dec;34:97-103. doi: 10.1016/j.mib.2016.08.005. Epub 2016 Sep 9. Review. PMID: 27614711 Free PMC Article
High-resolution analysis of multi-copy variant surface glycoprotein gene expression sites in African trypanosomes. Hutchinson S, Glover L, Horn D. BMC Genomics. 2016 Oct 18;17(1):806. PMID: 27756224 Free PMC Article
The role of folate transport in antifolate drug action in Trypanosoma brucei. Dewar S, Sienkiewicz N, Ong HB, Wall RJ, Horn D, Fairlamb AH. J Biol Chem. 2016 Nov 18;291(47):24768-24778. Epub 2016 Oct 4. PMID: 27703008 Free PMC Article
Co-dependence between trypanosome nuclear lamina components in nuclear stability and control of gene expression. Maishman L, Obado SO, Alsford S, Bart JM, Chen WM, Ratushny AV, Navarro M, Horn D, Aitchison JD, Chait BT, Rout MP, Field MC. Nucleic Acids Res. 2016 Dec 15;44(22):10554-10570. Epub 2016 Sep 12. PMID: 27625397 Free PMC Article
VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes. Glover L, Hutchinson S, Alsford S, Horn D. Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7225-30. doi: 10.1073/pnas.1600344113. Epub 2016 May 25. PMID: 27226299 Free PMC Article
How antiparasitic drugs work – and sometimes stop working! Horn D. PLoS Pathog. 2016 May 5;12(5):e1005430. doi: 10.1371/journal.ppat.1005430. eCollection 2016 May. No abstract available. PMID: 27149375 Free PMC Article
Epigenetic regulation of virulence gene expression in parasitic protozoa. Duraisingh MT, Horn D. Cell Host Microbe. 2016 May 11;19(5):629-40. doi: 10.1016/j.chom.2016.04.020. Review. PMID: 27173931 Free PMC Article
Comparative genomics of drug resistance in Trypanosoma brucei rhodesiense. Graf FE, Ludin P, Arquint C, Schmidt RS, Schaub N, Kunz Renggli C, Munday JC, Krezdorn J, Baker N, Horn D, Balmer O, Caccone A, de Koning HP, Mäser P. Cell Mol Life Sci. 2016 Sep;73(17):3387-400. doi: 10.1007/s00018-016-2173-6. Epub 2016 Mar 14. PMID: 26973180 Free PMC Article
Pentamidine Is not a permeant but a nanomolar inhibitor of the Trypanosoma brucei aquaglyceroporin-2. Song J, Baker N, Rothert M, Henke B, Jeacock L, Horn D, Beitz E. PLoS Pathog. 2016 Feb 1;12(2):e1005436. doi: 10.1371/journal.ppat.1005436. eCollection 2016 Feb. PMID: 26828608 Free PMC Article
2015
Modulation of the surface proteome through multiple ubiquitylation pathways in African trypanosomes. Zoltner M, Leung KF, Alsford S, Horn D, Field MC. PLoS Pathog. 2015 Oct 22;11(10):e1005236. doi: 10.1371/journal.ppat.1005236. eCollection 2015 Oct. PMID: 26492041 Free PMC Article
Vacuolar ATPase depletion affects mitochondrial ATPase function, kinetoplast dependency, and drug sensitivity in trypanosomes. Baker N, Hamilton G, Wilkes JM, Hutchinson S, Barrett MP, Horn D. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9112-7. doi: 10.1073/pnas.1505411112. Epub 2015 Jul 6. PMID: 26150481 Free PMC Article
Chimerization at the AQP2-AQP3 locus is the genetic basis of melarsoprol-pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates. Graf FE, Baker N, Munday JC, de Koning HP, Horn D, Mäser P. Int J Parasitol Drugs Drug Resist. 2015 May 7;5(2):65-8. doi: 10.1016/j.ijpddr.2015.04.002. eCollection 2015 Aug. PMID: 26042196 Free PMC Article
Trypanosoma brucei J protein 2 is a stress inducible and essential Hsp40. Ludewig MH, Boshoff A, Horn D, Blatch GL. Int J Biochem Cell Biol. 2015 Mar;60:93-8. doi: 10.1016/j.biocel.2014.12.016. Epub 2015 Jan 3. PMID: 25562516
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes. Glover L, Alsford S, Baker N, Turner DJ, Sanchez-Flores A, Hutchinson S, Hertz-Fowler C, Berriman M, Horn D. Nat Protoc. 2015 Jan;10(1):106-33. doi: 10.1038/nprot.2015.005. Epub 2014 Dec 11. PMID: 25502887
2014
Locus-specific control of DNA resection and suppression of subtelomeric VSG recombination by HAT3 in the African trypanosome. Glover L, Horn D. Nucleic Acids Res. 2014 Nov 10;42(20):12600-13. doi: 10.1093/nar/gku900. Epub 2014 Oct 9. PMID: 25300492 Free PMC Article
Antiparasitic chemotherapy: from genomes to mechanisms. Horn D, Duraisingh MT. Annu Rev Pharmacol Toxicol. 2014;54:71-94. doi: 10.1146/annurev-pharmtox-011613-135915. Epub 2013 Sep 11. Review. PMID: 24050701 Free PMC Article
Antigenic variation in African trypanosomes. Horn D. Mol Biochem Parasitol. 2014 Jul;195(2):123-9. doi: 10.1016/j.molbiopara.2014.05.001. Epub 2014 May 22. Review. PMID: 24859277 Free PMC Article
Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei. Alsford S, Currier RB, Guerra-Assunção JA, Clark TG, Horn D. PLoS Pathog. 2014 May 15;10(5):e1004130. doi: 10.1371/journal.ppat.1004130. eCollection 2014 May. PMID: 24830321 Free PMC Article
Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei. Mony BM, MacGregor P, Ivens A, Rojas F, Cowton A, Young J, Horn D, Matthews K. Nature. 2014 Jan 30;505(7485):681-685. doi: 10.1038/nature12864. Epub 2013 Dec 15. PMID: 24336212 Free PMC Article
Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs.Munday JC, Eze AA, Baker N, Glover L, Clucas C, Aguinaga Andrés D, Natto MJ, Teka IA, McDonald J, Lee RS, Graf FE, Ludin P, Burchmore RJ, Turner CM, Tait A, MacLeod A, Mäser P, Barrett MP, Horn D, De Koning HP. J Antimicrob Chemother. 2014 Mar;69(3):651-63. doi: 10.1093/jac/dkt442. Epub 2013 Nov 13. PMID: 24235095 Free PMC Article
High-throughput decoding of drug targets and drug resistance mechanisms in African trypanosomes. Horn D. Parasitology. 2014 Jan;141(1):77-82. doi: 10.1017/S0031182013000243. Epub 2013 Apr 8. Review. PMID: 23561654
Trypanosoma brucei subtelomeres: Monoallelic expression and antigenic variation. Figueiredo L, Horn D. Subtelomeres. Springer. 2014. Ch 7:137-152. Ed. Edward Louis.
2013
PPL2 translesion polymerase is essential for the completion of chromosomal DNA replication in the African trypanosome. Rudd SG, Glover L, Jozwiakowski SK, Horn D, Doherty AJ. Mol Cell. 2013 Nov 21;52(4):554-65. doi: 10.1016/j.molcel.2013.10.034. PMID: 24267450 Free PMC Article
Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol. Graf FE, Ludin P, Wenzler T, Kaiser M, Brun R, Pyana PP, Büscher P, de Koning HP, Horn D, Mäser P. PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2475. doi: 10.1371/journal.pntd.0002475. eCollection 2013. PMID: 24130910 Free PMC ArticleAntigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control. Glover L, Hutchinson S, Alsford S, McCulloch R, Field MC, Horn D. Cell Microbiol. 2013 Dec;15(12):1984-93. doi: 10.1111/cmi.12215. Epub 2013 Oct 10. Review. PMID: 24047558 Free PMC Article
Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA. Gould MK, Bachmaier S, Ali JA, Alsford S, Tagoe DN, Munday JC, Schnaufer AC, Horn D, Boshart M, de Koning HP. Antimicrob Agents Chemother. 2013 Oct;57(10):4882-93. doi: 10.1128/AAC.00508-13. Epub 2013 Jul 22. PMID: 23877697 Free PMC Article
Receptor-mediated endocytosis for drug delivery in African trypanosomes: fulfilling Paul Ehrlich’s vision of chemotherapy. Alsford S, Field MC, Horn D. Trends Parasitol. 2013 May;29(5):207-12. doi: 10.1016/j.pt.2013.03.004. Epub 2013 Apr 17. PMID: 23601931
DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. Glover L, Alsford S, Horn D. PLoS Pathog. 2013 Mar;9(3):e1003260. doi: 10.1371/journal.ppat.1003260. Epub 2013 Mar 28. PMID: 23555264 Free PMC Article
Genetic dissection of drug resistance in trypanosomes. Alsford S, Kelly JM, Baker N, Horn D. Parasitology. 2013 Oct;140(12):1478-91. doi: 10.1017/S003118201300022X. Epub 2013 Apr 3. Review. PMID: 23552488 Free PMC Article
Drug resistance in African trypanosomiasis: the melarsoprol and pentamidine story. Baker N, de Koning HP, Mäser P, Horn D. Trends Parasitol. 2013 Mar;29(3):110-8. doi: 10.1016/j.pt.2012.12.005. Epub 2013 Jan 30. Review. PMID: 23375541 Free PMC Article
2012
Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei. Alsford S, Horn D. Nucleic Acids Res. 2012 Nov 1;40(20):10150-60. doi: 10.1093/nar/gks813. Epub 2012 Aug 31. PMID: 22941664 Free PMC Article
Telomeres, tethers and trypanosomes. Field MC, Horn D, Alsford S, Koreny L, Rout MP. Nucleus. 2012 Nov-Dec;3(6):478-86. doi: 10.4161/nucl.22167. Epub 2012 Sep 19. PMID: 22992703 Free PMC Article
Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. Baker N, Glover L, Munday JC, Aguinaga Andrés D, Barrett MP, de Koning HP, Horn D. Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10996-1001. doi: 10.1073/pnas.1202885109. Epub 2012 Jun 18. PMID: 22711816 Free PMC Article
Epigenetic mechanisms, nuclear architecture and the control of gene expression in trypanosomes. Alsford S, duBois K, Horn D, Field MC. Expert Rev Mol Med. 2012 May 29;14:e13. doi: 10.1017/erm.2012.7. Review. PMID: 22640744
NUP-1 Is a large coiled-coil nucleoskeletal protein in trypanosomes with lamin-like functions. DuBois KN, Alsford S, Holden JM, Buisson J, Swiderski M, Bart JM, Ratushny AV, Wan Y, Bastin P, Barry JD, Navarro M, Horn D, Aitchison JD, Rout MP, Field MC. PLoS Biol. 2012;10(3):e1001287. doi: 10.1371/journal.pbio.1001287. Epub 2012 Mar 27. PMID: 22479148 Free PMC Article
Trypanosomal histone γH2A and the DNA damage response. Glover L, Horn D. Mol Biochem Parasitol. 2012 May;183(1):78-83. doi: 10.1016/j.molbiopara.2012.01.008. Epub 2012 Feb 14. PMID: 22353557 Free PMC Article
Inhibitors of human histone deacetylase with potent activity against the African trypanosome Trypanosoma brucei. Kelly JM, Taylor MC, Horn D, Loza E, Kalvinsh I, Björkling F. Bioorg Med Chem Lett. 2012 Mar 1;22(5):1886-90. doi: 10.1016/j.bmcl.2012.01.072. Epub 2012 Jan 28. PMID: 22326398 Free PMC Article
High-throughput decoding of antitrypanosomal drug efficacy and resistance. Alsford S, Eckert S, Baker N, Glover L, Sanchez-Flores A, Leung KF, Turner DJ, Field MC, Berriman M, Horn D. Nature. 2012 Jan 25;482(7384):232-6. doi: 10.1038/nature10771. PMID: 22278056 Free PMC Article
2011
High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Alsford S, Turner DJ, Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C, Horn D. Genome Res. 2011 Jun;21(6):915-24. doi: 10.1101/gr.115089.110. Epub 2011 Mar 1. PMID: 21363968 Free PMC Article
Elongator protein 3b negatively regulates ribosomal DNA transcription in african trypanosomes. Alsford S, Horn D. Mol Cell Biol. 2011 May;31(9):1822-32. doi: 10.1128/MCB.01026-10. Epub 2011 Feb 28. PMID: 21357738 Free PMC Article
Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6. Baker N, Alsford S, Horn D. Mol Biochem Parasitol. 2011 Mar;176(1):55-7. doi: 10.1016/j.molbiopara.2010.11.010. Epub 2010 Nov 18. PMID: 21093499 Free PMC Article
Microhomology-mediated deletion and gene conversion in African trypanosomes. Glover L, Jun J, Horn D. Nucleic Acids Res. 2011 Mar;39(4):1372-80. doi: 10.1093/nar/gkq981. Epub 2010 Oct 21. PMID: 20965968 Free PMC Article
2010
Molecular mechanisms underlying the control of antigenic variation in African trypanosomes. Horn D, McCulloch R. Curr Opin Microbiol. 2010 Dec;13(6):700-5. doi: 10.1016/j.mib.2010.08.009. Epub 2010 Sep 29. Review. PMID: 20884281 Free PMC Article
Histone deacetylases play distinct roles in telomeric VSG expression site silencing in African trypanosomes. Wang QP, Kawahara T, Horn D. Mol Microbiol. 2010 Sep;77(5):1237-45. doi: 10.1111/j.1365-2958.2010.07284.x. PMID: 20624217 Free PMC Article
RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease. Alsford S, Kemp LE, Kawahara T, Horn D. Mol Biochem Parasitol. 2010 Nov;174(1):70-3. doi: 10.1016/j.molbiopara.2010.06.006. Epub 2010 Jun 14. PMID: 20558212 Free PMC Article
2009
What has DNA sequencing revealed about the VSG expression sites of African trypanosomes? McCulloch R, Horn D. Trends Parasitol. 2009 Aug;25(8):359-63. doi: 10.1016/j.pt.2009.05.007. Epub 2009 Jul 23. PMID: 19632154
Antigenic variation: extending the reach of telomeric silencing. Horn D. Curr Biol. 2009 Jun 23;19(12):R496-8. doi: 10.1016/j.cub.2009.04.049. PMID: 19549499 Free Article
Site-specific DNA double-strand breaks greatly increase s transformation efficiency in Trypanosoma brucei. Glover L, Horn D. Mol Biochem Parasitol. 2009 Aug;166(2):194-7. PMID: 19459229 Free PMC Article
DNA breaks as triggers for antigenic variation in African trypanosomes. Alsford S, Horn D, Glover L. Genome Biol. 2009;10(6):223. doi: 10.1186/gb-2009-10-6-223. Epub 2009 Jun 8. Review. PMID: 19519956 Free PMC Article
2008
Two essential MYST-family proteins display distinct roles in histone H4K10 acetylation and telomeric silencing in trypanosomes. Kawahara T, Siegel TN, Ingram AK, Alsford S, Cross GA, Horn D. Mol Microbiol. 2008 Aug;69(4):1054-68. doi: 10.1111/j.1365-2958.2008.06346.x. Epub 2008 Jul 9. PMID: 18631159 Free PMC Article
Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei. Alsford S, Horn D. Mol Biochem Parasitol. 2008 Sep;161(1):76-9. doi: 10.1016/j.molbiopara.2008.05.006. Epub 2008 Jun 7. PMID: 18588918 Free PMC Article
Analysis of small GTPase function in trypanosomes. Field MC, Horn D, Carrington M. Methods Enzymol. 2008;438:57-76. doi: 10.1016/S0076-6879(07)38005-1. PMID: 18413241
A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes. Wilkinson SR, Taylor MC, Horn D, Kelly JM, Cheeseman I. Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5022-7. doi: 10.1073/pnas.0711014105. Epub 2008 Mar 26. PMID: 18367671 Free PMC Article
Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes. Glover L, McCulloch R, Horn D. Nucleic Acids Res. 2008 May;36(8):2608-18. doi: 10.1093/nar/gkn104. Epub 2008 Mar 11. PMID: 18334531 Free PMC Article
Acetylation of histone H4K4 is cell cycle regulated and mediated by HAT3 in Trypanosoma brucei. Siegel TN, Kawahara T, Degrasse JA, Janzen CJ, Horn D, Cross GA. Mol Microbiol. 2008 Feb;67(4):762-71. doi: 10.1111/j.1365-2958.2007.06079.x. Epub 2007 Dec 30. PMID: 18179414 Free PMC Article
Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids. Horn D. BMC Genomics. 2008 Jan 3;9:2. doi: 10.1186/1471-2164-9-2. PMID: 18173843 Free PMC Article
Histone deacetylases. Horn D. Adv Exp Med Biol. 2008;625:81-6. doi: 10.1007/978-0-387-77570-8_7. Review. PMID: 18365660
2007
Introducing histone modification in trypanosomes. Horn D. Trends Parasitol. 2007 Jun;23(6):239-42. Epub 2007 Apr 11. PMID: 17433777 Free PMC Article
RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei. Alsford S, Horn D. Mol Biochem Parasitol. 2007 May;153(1):77-9. Epub 2007 Jan 21. No abstract available. PMID: 17316839 Free PMC Article
Deletion of a trypanosome telomere leads to loss of silencing and progressive loss of terminal DNA in the absence of cell cycle arrest. Glover L, Alsford S, Beattie C, Horn D. Nucleic Acids Res. 2007;35(3):872-80. Epub 2007 Jan 23. PMID: 17251198 Free PMC Article
A sirtuin in the African trypanosome is involved in both DNA repair and telomeric gene silencing but is not required for antigenic variation. Alsford S, Kawahara T, Isamah C, Horn D. Mol Microbiol. 2007 Feb;63(3):724-36. Epub 2007 Jan 4. PMID: 17214740 Free Article
Deletion of the Trypanosoma brucei superoxide dismutase gene sodb1 increases sensitivity to nifurtimox and benznidazole. Prathalingham SR, Wilkinson SR, Horn D, Kelly JM. Antimicrob Agents Chemother. 2007 Feb;51(2):755-8. Epub 2006 Dec 4. PMID: 17145786 Free PMC Article
Chromosome structure and dynamics. Renauld H, Kelly JM, Horn D. In: Barry, D; Mottram, J; McCulloch, R; Acosta-Serrano, A; Renauld, H, (eds.) 2007. pp. 91-132. Trypanosomes – After the Genome. Horizon Scientific Press, Norwich, UK.,
2006
Chromosome-wide analysis of gene function by RNA interference in the African trypanosome. Subramaniam C, Veazey P, Redmond S, Hayes-Sinclair J, Chambers E, Carrington M, Gull K, Matthews K, Horn D, Field MC. Eukaryot Cell. 2006 Sep;5(9):1539-49. PMID: 16963636 Free PMC Article
Functional characterisation of the iron superoxide dismutase gene repertoire in Trypanosoma brucei. Wilkinson SR, Prathalingam SR, Taylor MC, Ahmed A, Horn D, Kelly JM. Free Radic Biol Med. 2006 Jan 15;40(2):198-209. Epub 2005 Aug 18. PMID: 16413403
Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres. Glover L, Horn D. EMBO Rep. 2006 Jan;7(1):93-9. PMID: 16311518 Free PMC Article
2005
Tagging a T. brucei RRNA locus improves s transfection efficiency and circumvents inducible expression position effects. Alsford S, Kawahara T, Glover L, Horn D. Mol Biochem Parasitol. 2005 Dec;144(2):142-8. Epub 2005 Sep 6. PMID: 16182389 Free PMC Article
The central roles of telomeres and subtelomeres in antigenic variation in African trypanosomes. Horn D, Barry JD. Chromosome Res. 2005;13(5):525-33. Review. PMID: 16132817
Vitamin C biosynthesis in trypanosomes: a role for the glycosome. Wilkinson SR, Prathalingam SR, Taylor MC, Horn D, Kelly JM. Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11645-50. Epub 2005 Aug 8. PMID: 16087875 Free PMC Article
The genome of the kinetoplastid parasite, Leishmania major. Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O’neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ. Science. 2005 Jul 15;309(5733):436-42. PMID: 16020728 Free PMC Article
The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B. Science. 2005 Jul 15;309(5733):409-15. PMID: 16020725 Free Article
Directional enrichment of directly cloned PCR products. Horn D. Biotechniques. 2005 Jul;39(1):40, 42, 44, 46. No abstract available. PMID: 16060367 Free Article
Down-regulating gene expression by RNA interference in Trypanosoma brucei. Clayton CE, Estévez AM, Hartmann C, Alibu VP, Field M, Horn D. Methods Mol Biol. 2005;309:39-60. No abstract available. PMID: 15990397
Multiplex analysis of RNA interference defects in Trypanosoma brucei. Alsford S, Glover L, Horn D. Mol Biochem Parasitol. 2005 Jan;139(1):129-32. No abstract available. PMID: 15610827 Free PMC Article
A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei. Alibu VP, Storm L, Haile S, Clayton C, Horn D. Mol Biochem Parasitol. 2005 Jan;139(1):75-82. PMID: 15610821
2004
The molecular control of antigenic variation in Trypanosoma brucei. Horn D. Curr Mol Med. 2004 Sep;4(6):563-76. Review. PMID: 15357208
2003
Trypanosomatid histones. Alsford S, Horn D. Mol Microbiol. 2004 Jul;53(2):365-72. Review. PMID: 15228519 Free Article
RNA interference identifies two hydroperoxide metabolizing enzymes that are essential to the bloodstream form of the african trypanosome. Wilkinson SR, Horn D, Prathalingam SR, Kelly JM. J Biol Chem. 2003 Aug 22;278(34):31640-6. Epub 2003 Jun 5. PMID: 12791697 Free Article
2002
Histone deacetylases in Trypanosoma brucei: two are essential and another is required for normal cell cycle progression. Ingram AK, Horn D. Mol Microbiol. 2002 Jul;45(1):89-97. PMID: 12100550 Free Article
2001
Nuclear gene transcription and chromatin in Trypanosoma brucei. Horn D. Int J Parasitol. 2001 Sep;31(11):1157-65. Review. PMID: 11513885
2000
Genetic manipulation indicates that ARD1 is an essential Na-acetyltransferase in Trypanosoma brucei. Ingram AK, Cross GA, Horn D. Mol Biochem Parasitol. 2000 Dec;111(2):309-17. PMID: 11163439
Telomere maintenance and length regulation in Trypanosoma brucei. Horn D, Spence C, Ingram AK. EMBO J. 2000 May 15;19(10):2332-9. PMID: 10811624 Free PMC Article
1997
Position-dependent and promoter-specific regulation of gene expression in Trypanosoma brucei. Horn D, Cross GA. EMBO J. 1997 Dec 15;16(24):7422-31. PMID: 9405371 Free PMC Article
Analysis of Trypanosoma brucei vsg expression site switching in vitro. Horn D, Cross GA. Mol Biochem Parasitol. 1997 Feb;84(2):189-201. PMID: 9084039
1995
A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei. Horn D, Cross GA. Cell. 1995 Nov 17;83(4):555-61. PMID: 7585958 Free Article
1994
Mice lacking Snrpn expression show normal regulation of neuronal alternative splicing events. Huntriss JD, Barr JA, Horn DA, Williams DG, Latchman DS. Mol Biol Rep. 1994 Jul;20(1):19-25. Review. PMID: 7845394
1993
The tissue specific SmN protein does not influence the alternative splicing of endogenous N-Cam and C-SRC RNAs in transfected 3T3 cells. Horn DA, Latchman DS. Brain Res Mol Brain Res. 1993 Aug;19(3):181-7. PMID: 8412559
A distal region of the CALC-1 gene is necessary for regulated alternative splicing. Horn DA, Latchman DS. FEBS Lett. 1993 Jun 14;324(2):123-6. PMID: 8508914 Free Article
Alternative splicing of the mRNA encoding the alpha subunits of the G(o) GTP-binding protein during brain development and in neuronal cell lines. Horn DA, Latchman DS. Neurosci Lett. 1993 May 28;155(1):57-60. PMID: 8361664
Expression of the SmN splicing protein is developmentally regulated in the rodent brain but not in the rodent heart. Grimaldi K, Horn DA, Hudson LD, Terenghi G, Barton P, Polak JM, Latchman DS. Dev Biol. 1993 Apr;156(2):319-23. PMID: 8462734
The cardiac form of the tissue-specific SmN protein is identical to the brain and embryonic forms of the protein. Gerrelli D, Grimaldi K, Horn D, Mahadeva U, Sharpe N, Latchman DS. J Mol Cell Cardiol. 1993 Mar;25(3):321-9. PMID: 8510173
1992
Redistribution of secretory granule components precedes that of synaptic vesicle proteins during differentiation of a neuronal cell line in serum-free medium. Wheatley SC, Suburo AM, Horn DA, Vucicevic V, Terenghi G, Polak JM, Latchman DS. Neuroscience. 1992 Dec;51(3):575-82. PMID: 1336820
Expression of the tissue specific splicing protein SmN in neuronal cell lines and in regions of the brain with different splicing capacities. Horn DA, Suburo A, Terenghi G, Hudson LD, Polak JM, Latchman DS. Brain Res Mol Brain Res. 1992 Nov;16(1-2):13-9. PMID: 1334191
Neuronal expression of the tissue specific splicing protein SmN and its possible role in pre mRNA splicing. Horn DA, Latchman DS. Biochem Soc Trans. 1992 Aug;20(3):266S. No abstract available. PMID: 1426556
Intracellular redistribution of neuropeptides and secretory proteins during differentiation of neuronal cell lines. Suburo AM, Wheatley SC, Horn DA, Gibson SJ, Jahn R, Fischer-Colbrie R, Wood JN, Latchman DS, Polak JM. Neuroscience. 1992;46(4):881-9. PMID: 1347412