FingerPrints Proteomics equipment and services

Services

The facility offers a range of services, including: 

• General Proteomics Services (sample preparation advice and gel analysis)
• Quantitative Proteomics Services (SILAC, TMT/TMTpro (6/10/16/18plex) and Label Free)
• Targeted MS based Analysis (PRM and MRM)
• Phosphorylation Site Mapping (Single Protein and Global Phosphoproteomics analysis)
• Ti 4+ IMAC HP /Zr 4+ IMAC HP Phosphopeptide enrichment
• Accurate Protein and Protein ± PTMs Mass Estimation (whole protein MW analysis)
• Protein Identification by 1D nanoLC-MS-MS
• General ESI-MS and MALDI-TOF-MS analysis 

Additional services

• SPS-MS3 (TMT) (Eclipse* and Fusion Tribrids) with RTS*
• DIA – Data Independent Acquisition (Astrals/Exploris 480 and QE+)
• Direct DIA Analysis
• Spectral Library Based DIA Analysis
• Hybrid Library Based DIA Analysis
• Additional in-depth data analysis: Interactive Volcano/MA plot 

Equipment

The facility benefits from 15 operational MS systems, including: 

• 2 Thermo Orbitrap Astral with Vanquish Neo UHPLC
• 1 Ascend Tribrid with Vanquish Neo UHPLC
• 1 Eclipse Tribrid (UVPD) with Vanquish Neo UHPLC
• 2 Fusion Tribrid (ETD) with RSLC Nano UHPLC
• 2 Exploris 480 with RSLC Nano UHPLC
• 1 Q-Exactive HF with RSLC Nano UHPLC
• 4 Q-Exactive Plus with RSLC Nano UHPLC
• 1 Bruker Autoflex Speed MALDI-TOF
• 1 Cyclic IMS with Acquity UHPLC for HDX

Laboratory automation

• 1 TECAN Resolvex 200 for automated S-trap sample preparation
• 3 ThermoFisher Preparative HPLC systems (automated high pH fractionation)
• 2 ThermoFisher KingFisher Robotic Prep Systems (Automated T4+IMAC, PAC and SP3)
• 1 Hamilton MICROLAB STARlet Liquid Handler