New blood test developed to tackle major livestock disease in Africa

The University of Dundee and the Global Alliance for Livestock Veterinary Medicines (GALVmed) have partnered to develop a simple and effective device that tests for a cattle disease that is endemic in 40 African countries and accounts for up to a 50 percent loss in milk and meat production in the continent.

The device, less than 3 inches long and similar in format to a pregnancy test, can identify within 30 minutes whether or not an animal is infected with the parasite Trypanosoma vivax from a single drop of blood. The simple device does not require electricity or any additional equipment, factors that are essential for deployment in resource-limited settings.

The cattle disease nagana, also called African animal trypanosomosis, is caused mainly by two parasite species, Trypanosoma vivax and Trypanosoma congolense, and affects huge swathes of sub-Saharan Africa. The T. vivax form of nagana has also spread to South America. With around 60 million cattle at risk from the disease, which causes muscle wasting and death, the socio-economic impacts of nagana are profound. It has an impact on the lives of millions of smallholder farmers and the economy.

“Nagana is difficult to diagnose because early symptoms can be easily confused with a myriad of other endemic diseases,” said Professor Mike Ferguson, Regius Professor of Life Sciences at the University of Dundee, who led the research team.

“There is an urgent need for new, inexpensive and simple, diagnostics that can be used by vets and farmers to test animals prior to deploying expensive medicines.”

With this in mind GALVmed asked the University of Dundee to help develop a new diagnostic for nagana.

GALVmed is an NGO which makes livestock vaccines, medicines and diagnostics accessible and affordable to the millions of smallholder farmers in developing world, headquartered in Edinburgh.

Taking a hi-tech approach, the research team identified the components of Trypanosoma vivax that cattle make antibodies to.

One of these components was developed into a prototype diagnostic device in collaboration with Dr Steven Wall (Product Support Manager) at BBI Solutions OEM Limited, who specialise in the development and manufacturing of lateral flow assays in the Dundee MediPark.

The prototype diagnostic device was evaluated with over a hundred serum samples from uninfected and T. vivax-infected cattle. The promising results, just published in the journal PLOS Neglected Tropical Diseases, have inspired GALVmed to further investigate this innovative diagnostic test for use in Africa.

Dr Jeremy Salt, Senior Director of Research and Development at GALVmed, said, “We are delighted with the progress on an effective and simple diagnostic test for T. vivax infection through our partnership with The University of Dundee and BBI Solutions OEM Limited.

“Such a test could allow millions of smallholder farmers an efficient way to test their cattle for this debilitating disease and give peace of mind that any subsequent treatment for T. vivax infection will be done with the certainty that the patient is infected, which saves the farmer money. This will give more control to the smallholder farmers whose quality of life has been affected by this disease that covers over 10 million square kilometres of Africa.

“To ensure that the final test is widely used throughout the regions where it's endemic, GALVmed will be working with scientists, manufacturers and distributors in the 40 countries where AAT is rife to create a sustainable supply chain for the final product.”

Professor Ferguson added, “I am very proud of the talented scientists, Jennifer Fleming, Lalitha Sastry, Lauren Sullivan and Steven Wall, who did this work, and of the synergistic relationship between the University of Dundee and BBI Solutions OEM Limited. Hopefully, with further development by GALVmed, the device will prove sufficiently useful to be adopted for the detection of nagana caused by T. vivax in the developing world.”

The paper in PLOS Neglected Tropical Diseases can be viewed here:

http://dx.plos.org/10.1371/journal.pntd.0004977

Notes to Editors

The University of Dundee is the top ranked University in the United Kingdom for biological sciences, according to the 2014 Research Excellence Framework, the main test of research quality across all of the UK’s universities. Medical research at Dundee was ranked best in the UK for impact.

Dundee is the Scottish University of the Year 2016 (The Times & Sunday Times) and is internationally recognised for the quality of its teaching and research.

The University is the central hub for a multi-million pound biotechnology sector in the east of Scotland, which now accounts for 16% of the local economy. www.dundee.ac.uk

GALVmed, through its partners, makes livestock vaccines, medicines and diagnostics accessible and available to the millions of smallholder livestock and poultry keepers in developing countries for whom livestock is a lifeline.

The international NGO harnesses the best available expertise and capabilities to develop vaccines, medicines and diagnostics for neglected livestock diseases impacting smallholders in Africa and South Asia. For more information, please visit www.galvmed.org.

BBI Solutions is a leading manufacturer of raw materials and finished test platforms for the in-vitro diagnostics market. BBI is a global business with manufacturing sites spanning three countries.

Our range of raw materials includes human antigens, antibodies, serum and plasma products and clinical chemistry enzymes. We manufacture world renowned labels for lateral flow, ELISA assay and biosensors, including our gold nanoparticles and glucose oxidase.

BBI Solutions offer contract development and manufacturing services with a core focus on gold conjugation and lateral flow test manufacture.

BBI Solutions is part of the BBI Group.

www.bbisolutions.com


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