Celine obtained her PhD from University of Lyon (France) in 2003 whilst working on the microenvironment of bowel cancer with Pr. J.Y. Scoazec. From 2003-2005, she was a postdoctoral fellow at the University of Lyon with Pr. Scoazec and Dr. P. Rousselle. Since 2005, she is a post-doctoral fellow in Dr. A. South’s laboratory which is based at Ninewells Hospital in the Division of Cancer Research. Celine is investigating the different factors involved in cutaneous cancer cell migration and invasion. She is also developing mouse models of skin cancer and genetic skin disorders to test therapeutic compounds. Celine is funded by an ERC grant.
Metastasis is the process where cancer cells disseminate and establish secondary tumours at distant sites and is acknowledged to be responsible for cancer deaths in most cases. During this process, tumour cells which have already acquired proliferative advantages complete their progression by increasing their migratory capability enabling them to disseminate and establish secondary tumours at distant sites. Using gene expression profiling, Dr. A. South identified actin-related proteins as being potentially involved in cutaneous squamous cell carcinoma (cSCC) keratinocyte motility in response to stimuli from the microenvironement. Celine is currently investigating this hypothesis using 2D or 3D co-culture models of cutaneous cancer cell migration and invasion.
Celine is also strongly involved in Dr. A. South’s lab in vivo work. Potential anti-cSCC targets were identified by gene expression profiling array and were validated in vitro. With Sheila Wright, Celine is testing drugs against one of these anti-cSCC targets in a mouse model of cSCC xenograft. She is also strongly involved in the development of xenotransplantation that consist in grafting a skin equivalent on the back of SCID mice. This model will be used as preclinical model of skin cancer and genetic skin disease therapies.
C. Pourreyron, M. Chen, J.A. McGrath, J.C. Salas-Alanis, A.P. South, I.M. Leigh: High levels of type VII Collagen expression in RDEB cSCC Keratinocytes increases PI3K and MAPK Signalling, Cell Migration and Invasion, Br J Dermatol in press.
Sokolovski SG, Zolotovskaya SA, Goltsov A, Pourreyron C, South AP, Rafailov EU: Infrared laser pulse triggers increased singlet oxygen production in tumour cells, Sci Rep 2013, 3:3484
Michael M, Begum R, Fong K, Pourreyron C, South AP, McGrath JA, Parsons M: BPAG1-e Restricts Keratinocyte Migration through Control of Adhesion Stability, J Invest Dermatol 2013,
Kluk MJ, Ashworth T, Wang H, Knoechel B, Mason EF, Morgan EA, Dorfman D, Pinkus G, Weigert O, Hornick JL, Chirieac LR, Hirsch M, Oh DJ, South AP, Leigh IM, Pourreyron C, Cassidy AJ, Deangelo DJ, Weinstock DM, Krop IE, Dillon D, Brock JE, Lazar AJ, Peto M, Cho RJ, Stoeck A, Haines BB, Sathayanrayanan S, Rodig S, Aster JC: Gauging NOTCH1 Activation in Cancer Using Immunohistochemistry, PLoS One 2013, 8:e67306
Dayal JH, Cole CL, Pourreyron C, Watt SA, Lim YZ, Salas-Alanis JC, Murrell DF, McGrath JA, Stieger B, Jahoda C, Leigh IM, South AP: Type VII collagen regulates tumour expression of organic anion transporting polypeptide OATP1B3, promotes front to rear polarity and increases structural organisation in 3D spheroid cultures of recessive dystrophic epidermolysis bullosa tumour keratinocytes, J Cell Sci 2013,
Pourreyron C, Reilly L, Proby C, Panteleyev A, Fleming C, McLean K, South AP, Foerster J: Wnt5a is strongly expressed at the leading edge in non-melanoma skin cancer, forming active gradients, while canonical Wnt signalling is repressed, PLoS One 2012, 7:e31827
Ng YZ, Pourreyron C, Salas-Alanis JC, Dayal JH, Cepeda-Valdes R, Yan W, Wright S, Chen M, Fine JD, Hogg FJ, McGrath JA, Murrell DF, Leigh IM, Lane EB, South AP: Fibroblast-derived dermal matrix drives development of aggressive cutaneous squamous cell carcinoma in patients with recessive dystrophic epidermolysis bullosa, Cancer Res 2012, 72:3522-3534
McGrath JA, Stone KL, Begum R, Simpson MA, Dopping-Hepenstal PJ, Liu L, McMillan JR, South AP, Pourreyron C, McLean WH, Martinez AE, Mellerio JE, Parsons M: Germline Mutation in EXPH5 Implicates the Rab27B Effector Protein Slac2-b in Inherited Skin Fragility, Am J Hum Genet 2012, 91:1115-1121
Clements SE, Techanukul T, Lai-Cheong JE, Mee JB, South AP, Pourreyron C, Burrows NP, Mellerio JE, McGrath JA: Mutations in AEC syndrome skin reveal a role for p63 in basement membrane adhesion, skin barrier integrity and hair follicle biology, Br J Dermatol 2012, 167:134-144
Watt SA, Pourreyron C, Purdie K, Hogan C, Cole CL, Foster N, Pratt N, Bourdon JC, Appleyard V, Murray K, Thompson AM, Mao X, Mein C, Bruckner-Tuderman L, Evans A, McGrath JA, Proby C, Foerster J, Leigh IM, South AP: Integrative mRNA profiling comparing cultured primary cells with clinical samples reveals PLK1 and C20orf20 as therapeutic targets in cutaneous squamous cell carcinoma, Oncogene 2011, 30:4666-4677
Walter T, Hommell-Fontaine J, Gouysse G, Pourreyron C, Nejjari M, Villaume K, Causeret S, Hervieu V, Poncet G, Roche C, Scoazec JY: Effects of somatostatin and octreotide on the interactions between neoplastic gastroenteropancreatic endocrine cells and endothelial cells: a comparison between in vitro and in vivo properties, Neuroendocrinology 2011, 94:200-208
Purdie KJ, Pourreyron C, South AP: Isolation and culture of squamous cell carcinoma lines, Methods Mol Biol 2011, 731:151-159
Pourreyron C, Purdie KJ, Watt SA, South AP: Feeder layers: co-culture with nonneoplastic cells, Methods Mol Biol 2011, 731:467-470
Patel DC, Albrecht C, Pavitt D, Paul V, Pourreyron C, Newman SP, Godsland IF, Valabhji J, Johnston DG: Type 2 diabetes is associated with reduced ATP-binding cassette transporter A1 gene expression, protein and function, PLoS One 2011, 6:e22142
Purdie KJ, Pourreyron C, Fassihi H, Cepeda-Valdes R, Frew JW, Volz A, Weissenborn SJ, Pfister H, Proby C, Bruckner-Tuderman L, Murrell DF, Salas-Alanis JC, McGrath JA, Leigh IM, Harwood CA, South AP: No evidence that human papillomavirus is responsible for the aggressive nature of recessive dystrophic epidermolysis bullosa-associated squamous cell carcinoma, J Invest Dermatol 2010, 130:2853-2855
Poncet G, Villaume K, Walter T, Pourreyron C, Theillaumas A, Lepinasse F, Hervieu V, Cordier-Bussat M, Scoazec JY, Roche C: Angiogenesis and tumor progression in neuroendocrine digestive tumors, J Surg Res 2009, 154:68-77
Pourreyron C, Poncet G, Roche C, Gouysse G, Nejjari M, Walter T, Villaume K, Jacquier MF, Bernard C, Dumortier J, Chayvialle JA, Bachelot T, Scoazec JY: The role of angiogenesis in endocrine liver metastases: an experimental study, J Surg Res 2008, 144:64-73