Professor Aleksandar Jovanovic

There are several research lines that are focus of Cellular Signalling laboratory. This would be: 1) identifying intracellular signalling pathways involved in cellular defence against different forms of stress, 2) understanding structure, function and regulation of some ion channels, in particular ATP-sensitive K+ channels, 3) understanding aetio-pathogenesis of channelopathies and their functional manifestations.

To address these scientific questions we apply state-of-the-art physiological and biochemical methods, including patch clamp electrophysiology, digital epifluorescent imaging and laser confocal microscopy, recombinant and transgenic technologies, protein biochemistry, analytical biochemistry and others. Some examples of work done in this laboratory are presented on panels below.

Some examples of work done in Cellular Signalling Laboratory

Left up. Laser confocal images of rounded cardiomyocytes stained with either anti-Kir6.2 (upper image) or anti-SUR2A (lower image) and anti-LDH antibodies labelled with rhodamine (red channel) or fluorescein (green channel). Yellow colour is indicative of co-localization. Left down. Western blotting with anti-LDH antibody of anti-SUR2A or anti-Kir6.2 immunoprecipitate from A549 cells transfected with Kir6.2 and SUR2A (alone and combined) plus N( N)- or C( C)-termini deletion mutants of M-LDH. Right up (middle). Upper: Recording of BKCa channel activity in membrane patch excised from alveolar epithelial A549 cell under normoxia (PO2=140 mm Hg) and hypoxia (PO2=20 mm Hg). Lower: Recording of single KATP channel activity in membrane patch excised from ventricular cardiomyocyte. Right down. Images of cardiomyocytes loaded with Ca2+-sensitive day, Fluo-3, under control condition (left image) and after ischaemia/reperfusion (right image). For more details concerning these images, recordings and blots see Ranki et al., 2001, J Am Coll Cardiol 38: 906-915; Crawford et al., 2002, EMBO J 21: 3936-3948 and Jovanovic et al., 2003, Am J Respir Cell Mol Biol 28: 363-372.

Publications

Maintained by: H Murphie.

The University of Dundee is a Scottish Registered Charity, No.SC015096

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